Reporters learned from the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences on the 23rd that researchers from the Institute and the Center for Excellence and Innovation in Brain Science and Intelligent Technology of the Chinese Academy of Sciences have developed a set of efficient combination technology strategies, which for the first time simultaneously overcome two major epigenetic barriers faced in the development of somatic cloned embryos, set the world's highest record for the efficiency of mouse somatic cloning, and provided a practical technical strategy for efficient mammalian somatic cloning. The relevant research results were published in the journal Advanced Science. In this study, researchers successfully solved two major challenges in the development of cloned embryos through a combination of techniques. Firstly, they administered a special drug to the embryo and injected two mRNA encoding "modifying enzymes" that can remove epigenetic disorders regulated by genes, effectively overcoming developmental disorders of the embryo before implantation (in the uterus). At the same time, they cleverly used the "tetraploid compensation technology" to replace defective cells in cloned embryos that would develop into placenta in the future, thus solving the developmental stagnation caused by placental problems after implantation (in the uterus). This combination strategy has a significant effect, with a proportion of about 30% of transplanted embryos ultimately developing into healthy mice. Lu Falong, the corresponding author of the paper and a researcher at the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, said that this research, for the first time in a single cloned embryo, has solved two key epigenetic obstacles that hinder development before and after implantation, greatly improved the overall success rate of mammal Klong, and has a good guiding significance for efficient cloning of large mammals in the future. (New Society)